Table 1. The 14 SSR primers developed in this study.
| SSR locus | Product size (bp) | Motif | Forward primer a | Reverse primer a |
|---|---|---|---|---|
| 02 | 188–232 | (GAA)4 | ACGACGTTGTAAAAGCAATTAGACGGGGAGAGGA | GTTTCTTTTCCCATTACTGCCGTAGACATCCCCATT |
| 05 | 218–254 | (TGA)6 | ACGACGTTGTAAAACCTGAACGAAAGGATGCAGTT | GTTTCTTTTCCCATTAGGTGGCTCGTTGTCAGGTATG |
| 07 | 132–150 | (ATG)6 | ACGACGTTGTAAAATCGCCGACAACAGTAACGAC | GTTTCTTTTCCCATTAGCACGACCAACACCATCATT |
| 08 | 360–375 | (AAG)6 | ACGACGTTGTAAAAGCAGACGAAATTGCCTTTCA | GTTTCTTTTCCCATTACGGCGGGTATATTCTGTGGT |
| 12 | 288–330 | (CGA)5 | ACGACGTTGTAAAATCGAGTCGGATCACTTCGTC | GTTTCTTTTCCCATTAAGGAGCAGATGGATGGTGGT |
| 16 | 261–320 | (AG)16 | ACGACGTTGTAAAATGGTGAGGAGGATGGAAAGG | GTTTCTTTTCCCATTATTGATTGAACAAGTGAACAACAGC |
| 19 | 326–361 | (CA)12 | ACGACGTTGTAAAAGACGCCTTTATCGTTTGAGGA | GTTTCTTTTCCCATTAGGGATAGACACCGCTGAAGG |
| 27 | 308–342 | (ATG)11 | ACGACGTTGTAAAATTTGGACAGGACGAGCTTGA | GTTTCTTTTCCCATTATCCAAGGCCGCTTTACTCAT |
| 28 | 205–238 | (ATA)6 | ACGACGTTGTAAAACCTGATTTGGATGCGGGTAT | GTTTCTTTTCCCATTACCACCAGTCCCACCGATAAT |
| 29 | 228–268 | (ACA)9 | ACGACGTTGTAAAATACCCCAGTCCCGTCAAAAC | GTTTCTTTTCCCATTAAGCGAGAGTGCCTCCTGTTC |
| 31 | 147–226 | (AT)11 | ACGACGTTGTAAAATGTACGTGTCCCAGATCCTGA | GTTTCTTTTCCCATTATCAAATGAGGGCGGGATATT |
| 51 | 196–257 | (AT)7 | ACGACGTTGTAAAAGCTTTTGGTCAACGGTTTCG | GTTTCTTTTCCCATTATGACCATAGAAAGCCCCATGT |
| 58 | 180–227 | (ACA)12 | ACGACGTTGTAAAAGACGTTAACGGCAACCAGAA | GTTTCTTTTCCCATTAAGCGTTAGCACCACCGTTA |
| 61 | 304–332 | (TGC)7 | ACGACGTTGTAAAATGTTGGCCTGGTTGATTCTG | GTTTCTTTTCCCATTAGGCACAAACACCGCAAATAA |
a For genotyping by multiplex-ready PCR, a PIG-tail tag sequence was added to the 5’ end of each SSR primer: 5'-ACGACGTTGTAAAA for the forward primer and 5'-GTTTCTTTTCCCATTA for the reverse primer.