| pPlaTmp |
pPla plasmid of Y. pestis CO92 containing a dfr cassette; TmpR
|
[21] |
| pPlaD206A |
pPlaTmp with a nucleotide mutation at position 206 that abrogates the proteolytic activity of Pla |
This study |
| pGP704N-Km |
Suicide vector, AmpR, KmR
|
[43] |
| pPlaKm |
pPlaTmp plasmid containing a kanamycin cassette inserted into the dfr gene; KmR, TmpS. |
This study |
| pKOBEG-sacB |
repA cat araC pBAD exo bet gam sacB, CmR
|
[42] |
|
Y. pestis
|
|
|
| Yp.wt |
Wild type Y. pestis strain 6/69, biotype Orientalis |
[50] |
| Yp(ΔPla) |
6/69 cured of the pPla plasmid |
[43] |
| Yp(PlaD206A) |
pPlaD206A introduced into Yp(ΔPla) by electroporation |
This study |
| Yp(pKOBEG-sacB) (pPlaTmp) |
Y. pestis CO92 strain (biotype Orientalis) containing the pKOBEG-sacB and the pPlaTmp plasmid |
[21] |
| Yp(PlaD206A)(pPlaKm) |
Yp(PlaD206A) complemented with pPlaKm to restore the proteolytic activity of Pla |
This study |
|
Y. pseudotuberculosis
|
|
|
| Yptb* |
Y. pseudotuberculosis IP32953 in which O-antigen expression has been abrogated by deletion of the gmd and fcl loci. |
[21] |
| Yptb*(Pla) |
pPlaTmp introduced into Yptb* by electroporation |
[21] |
