Figure 2.

Representation of the high-throughput screen to quantify relative DGC and PDE activity. (A) To determine DGC activity in response to bile, individual DGCs are expressed from an IPTG-inducible vector in V. cholerae. Relative c-di-GMP synthesis is determined using a separate reporter vector containing the lux operon under the control of a c-di-GMP inducible promoter (6:C9-lux). Luminescence from each strain was measured in the presence and absence of bile. (B) To determine relative PDE activity, the DGC qrgB is expressed alongside individual PDEs from IPTG-inducible vectors. The same c-di-GMP lux reporter vector is measured in the presence and absence of bile to determine relative c-di-GMP hydrolysis.