Figure 2.

Intracellular distribution of peptide tagged GFP tracer proteins in living cells. (A) Representative microscopic images of live C2C12 cells transfected with GFP, NLS-GFP and a fusion of NLS-GFP with 8 glycines (8G) as example for the addition of neutral amino acids is shown in the first panel. The second panel shows images of cells with fusions of an increasing number of aspartates as examples for acidic amino acid fusions to NLS-GFP. The GFP fluorescence is depicted in the upper row with the corresponding differential interference contrast (DIC) images below. (B) Microscopic Images of the distribution of the NLS-GFP fused to 4 to 7 additional arginines. The graphs in (C) represent the mean distribution of the GFP constructs depicted in (A) and (B) in cytoplasm and nucleoli in relation to the nucleoplasm for an average of 10 cells from 2 independent experiments. Scalebars: 5 µm.