Figure 4.

Electrophoretic separation of the PCR products corresponding to the circRNAs of the SGMS1 gene. Electrophoretic separation of the PCR products corresponding to circRNAs was carried out after 40 amplification cycles. For the amplification, outward-facing primers corresponding to the sequences of exons 2 (A), 5 (B) and 7 (C) were used. (D) For the control of the amount of RNA in a sample, the level of the coding transcript of the SGMS1 gene was assessed using the inward-facing F1/F3 primers (28 amplification cycles). circRNAs are shown to the right of the electrophoretogram, and the lengths of PCR products are shown to its left (nt); tissues from which RNA was isolated are shown at the bottom.