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. 2015 Jan 13;11(10):1291–1300. doi: 10.1080/15476286.2014.996085

Figure 6.

Figure 6.

Analyses of GCase and LIMP-2 levels and GCase secretion into the extracellular environment. (A) Western blot shows a reduced amount of GCase after transfection with GBA siRNA and miR-127–5p compared to the NegCtrl siRNA. Conversely, GCase amount is increased in cells treated with miR-16–5p and miR-195–5p. LIMP-2 levels are decreased by miR-127–5p. The lower graphs indicate the percentage of relative density of each band normalized to the corresponding GAPDH band. (B) Evaluation of conditioned media shows GCase secretion (arrow) into the extracellular environment after transfection with miR-127–5p, compared to cells treated with NegCtrl siRNA. (C) Corresponding cell lysates from the conditioned media experiment show reduced GCase protein levels in N370S/N370S cells transfected with miR-127–5p. The bottom graph represents the intensity of each band normalized to the corresponding GAPDH band. For the relative density calculations in both (A) and (C), NegCtrl was considered as 100%.