Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 23;11(12):1568–1585. doi: 10.4161/15476286.2014.992280

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Author(s). Published with license by Taylor & Francis Group, LLC

© Kirk W Gaston and Patrick A Limbach

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

PMC Copyright notice

Figure 2. — Structural determination of N394. (A) The CID spectra of unlabeled (top) and deuterium (D)-labeled (bottom) ct⁶A bases (BH₂⁺). The product ions are assigned in the chemical structures of the ct⁶A base. D-labeled product ions are shown in red. Unassigned spectra containing the D-labeled portion are indicated by asterisks. (B) LC/MS coinjection of the synthetic and natural ct⁶A. UV traces (254 nm) and mass chromatograms (m/z 395) of synthetic ct⁶A (top), natural ct⁶A in E. coli total RNA (middle) and co-injected natural and synthetic ct⁶A (bottom). ct⁶A peaks in the UV trace are indicated by red arrows. (C) The chemical structures of ct⁶A and t⁶A are mutually convertible by hydration and dehydration. Reprinted by permission from Macmillan Publishers Ltd: Nature Chemical Biology, volume 9, pages 105–111 (2013).