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. 2015 Mar 4;6(1):66–76. doi: 10.1080/19491034.2015.1004256

Figure 2.

Figure 2.

Pericentric heterochromatin and epigenetic modifications of chromatin in blebs. (A) p.S143F fibroblasts (p17) were stained for LA/C, LB1 and centromeres. Hoechst was used to visualize DNA. The maximum projection of series of z-sections covering the entire nucleus are shown. A single centromere spot is found in the bleb region (arrow). Scale bar 5 μm. (B) p.S143F fibroblast (p17) stained for LA/C and acetylated histone H3 (AcetH3). A single mid-plane confocal section is shown. The intensity for AcetH3 is increased in the bleb region (arrow). Scale bar 5 μm. (C) p.S143F fibroblast (p17) stained for LA/C and histone H3 trimethylated at lysine 9 (H3K9me3). A single mid-plane confocal section is shown. H3K9me3 is reduced in the bleb region (arrow). Scale bar 5 μm.