Figure 3.

Venous thrombus age and the development of a CD31-positive luminal neoendothelial cell barrier layer. Fluorescence microscopy of VT sections was performed on mice after an intravenous injection of a fluorescent anti-mouse CD31 antibody. In a representative day 1 VT, minimal CD31 signal (magenta pseudocolor) overlies the thrombus, while in contrast a representative day 4 VT shows a thrombus-encased surface of CD31-positive neoendothelial signal at the luminal surface. H&E sections (dashed yellow line) and autofluorescence (AF, green pseudocolor) delineate the vein, as well as the lumen (*) and thrombosis (T). CD31-positive endothelial cells line the non-thrombosed venous lumen in both day 1 and day 4 animals. Scale bar, 50 µm.