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. 2015 Oct 6;5(12):1328–1342. doi: 10.7150/thno.11432

Figure 5.

Figure 5

Analysis of TNF-α producing cells. TNF-α expression in tumor tissue at 2 dpi was examined as described in the legend to Figure 4, except cells were stained for TNF-α (red) rather than IL-1β. Representative images from three independent experiments are shown (A-C). DAPI was used as a control. Scale bar = 100 µm. (D, E) Cells were re-stimulated with PMA and ionomycin in the presence of GolgiStop and the TNF-α+ cell population examined by intracellular staining. Multi-color analysis was used to examine the proportion of TNF-α+ cells within the total cell, macrophage (CD11b+F4/80+), neutrophil (CD11b+Gr1+), and dendritic cell (CD11c+MHCII+) populations. The indicated values represent the TNF-α+ population in the tumor-draining lymph nodes (D) and spleen (E). Data are representative of two individual experiments, each with similar results.