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. 2015 Oct 8;5(12):1363–1377. doi: 10.7150/thno.13181

Figure 2.

Figure 2

Autophagic flux was induced by PAMAM dendrimers in PC-12 cells. (A) Cells were plated in cell culture dishes with glass bottom. After treated with 10 μg/mL of PAMAM dendrimers G5 for 6h, 12h, 24h, and 36h, cell samples were stained with Cyto-ID Green autophagy dye and Lysotracker Red dye and analyzed by confocal microscopy. Untreated cells were used as negative control. (B) After exposure to 10μg/mL of PAMAM dendrimers G5 for indicated times, PC-12 cells were treated with or without 20 nM of Baf A1 for another 3 h. Changes in the expression of LC3 and p62 were examined by Western blot. (C) After exposure to a series of concentrations of PAMAM dendrimers for 24 h, PC-12 cells were treated with or without 20 nM of Baf A1 for another 3 h. Changes in expression of LC3 and p62 were examined by Western blot. (D) After exposure to 10μg/mL of PAMAM dendrimers G5 for indicated times, PC-12 cells were treated with or without 20 nM of Baf A1 for another 3 h. Changes in the expression of p62 was examined by Western blot. Cells treated by 10 nM of rapamycin for 24 h were as positive control. (E) After exposure to a series of concentrations of PAMAM dendrimers for 24 h, PC-12 cells were treated with or without 20 nM of Baf A1 for another 3 h. Changes in expression of p62 were examined by Western blot. Cells treated by 10 nM of rapamycin for 24 h were as positive control.