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. 2015 Oct 8;5(12):1363–1377. doi: 10.7150/thno.13181

Figure 3.

Figure 3

Suppression of reactive oxygen species impaired PAMAM dendrimers-induced autophagic effects. (A) PC-12 cells were incubated with or without PAMAM dendrimers in the presence or absence of antioxidants for 24h. Cell samples were treated with DCFH-DA and analyzed by microplate reader to quantify levels of ROS. *p<0.05 versus Ctrl, and #p<0.05 versus PAMAM dendrimers G5-treated group. (B) PC-12 cells were incubated with or without PAMAM dendrimers in the presence or absence of antioxidants for 24h. Cell samples were stained with Cyto-ID Green dye and MitoSox Red dye and analyzed by confocal microscopy. (C) and (D)The relative oxidative fluorescence and autophagic fluorescence intensity were quantified by confocal microscopy software. All data were presented as the means ± SD of more than three samples. *p<0.05 versus Ctrl, or versus PAMAM dendrimers G5-treated group. (E) PC-12 cells were treated as was described in (A). The whole protein was extracted, and LC3 was analyzed by Western blot.