Figure 1.

Efficiency of model monocistronic (A) CER mRNA translation relative to the control RFP mRNA translation efficiency or second cistron CER relative to control first cistron RFP in CER-RFP bicistronic (B) mRNA in the wild type strain (white bars) and ΔyciH strain (black bars). Schematic representation of mRNA CER is presented on the left panel, while translation efficiencies are shown on the right panel. All constructs' designations are indicated next to the schematic representations. Translation efficiencies of the CER reporter were normalized to the reference RFP construct. Exact values of relative translation efficiencies are shown next to the corresponding bars.