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. 2015 Mar 31;12(1):92–100. doi: 10.1080/15476286.2015.1017204

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Figure 1. — Canonical (Dicer-mediated) and non-canonical (AGO-2 mediated) paths for shRNA and miRNA processing (A) Canonical Dicer-mediated processing of shRNAs and pre-miRNAs. Top: a regular short hairpin RNA (shRNA) of 21 bp in length is processed by Dicer into siRNAs. Bottom: a regular pre-miRNA is processed by Dicer into the mature miRNA duplex. The guide strand (thick line) of the siRNA/miRNA duplex will subsequently be incorporated into RISC to induce RNAi silencing. (B) Non-canonical AGO2-mediated processing of AgoshRNAs and miR-451. Top: AgoshRNAs are hairpins of only 19 bp with a small terminal loop that bypass Dicer-processing and undergo cleavage by AGO2 between bp 10 and 11 on the 3′ side of the duplex (◂). Bottom: miR-451 is the founding member of a class of Dicer-independent, AGO2-processed miRNAs. A thick line represents the guide strand. Hairpin characteristics shared with the AgoshRNA design are the short stem and small loop. The miR-451 is processed further by tailing and trimming as indicated.