Figure 8.

AgoshRNA activity in Dicer-deficient cells HCT-116 cells that express the wt Dicer protein and a non-functional Dicer protein (Dcr-) were transfected with the regularly Dicer-processed shRNA construct (21/5) or the AGO2-processed AgoshRNA construct (19/5) together with a luciferase reporter encoding the sense or antisense target sequence, respectively. Activity scored in transfection of the luciferase reporter with the control plasmid pBS was set at 100%. The averages and standard deviations of 3 independent transfections are shown.