Figure 3.

(See previous page). Comparison of painted chromosome territories in nuclei of bovine fetal fibroblasts and cloned embryos. Panels A–D. Midplane nuclear sections from imaging stacks recorded with CLSM from a bovine fetal fibroblast nucleus (panel A), an ENP (panel B), an ENP/C (panel C) and an ENC (panel D) from cloned embryos after 3D-FISH with a BTA 13 paint probe (green). Chromosome painting demonstrates that individual CTs 13 correspond to individual major chromatin bodies (MCBs) with variable configurations, occasionally including nearby chromatin clusters (arrow). Three further MCBs painted with the BTA 13 probe were detected in other light optical sections from this nucleus (see Supplementary Fig. S1 in accompanying article by Popken et al.³⁵). Bars: 3 μm for A1–3, B1, B2, C1, C2, D1, D2; 2 μm for the enlarged box in A1 and for B3, B4; 1 μm for C3, C4.