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. 2015 Mar 31;12(3):305–319. doi: 10.1080/15476286.2015.1017233

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Figure 8. — Expression of TY-tagged EIF4G4 and variants in procyclic cells. Growth curves and Western blot analysis of the expression of the TY-EIF4G4 wild-type (A) or the TY-EIF4G4 LNK94-96AAA (B) and TY-EIF4G4 LL27-28AA (C) variants in transfected cells as shown for Fig. 7. (D) Interaction profile of wild-type TY-EIF4G4, or selected variants, with its EIF4AI and EIF4E3 binding partners. (E) Expression of EIF4G4-eYFP in transfected cells after 48 h of tetracycline induction in comparison with the endogenous protein in control cells.