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. 2015 Apr 29;6(3):202–206. doi: 10.1080/19490976.2015.1034417

Figure 1.

Figure 1.

The gut microbiota does not change TLR5 transcript levels and TLR5 expression does not depend on intact TLR2 or TLR4 signaling in the small intestine. (A) Small intestinal TLR5 mRNA levels of 10–14 week old male GF and CONV-R C57BL/6 mice (N = 6–8 mice per group) were quantified by qRT-PCR relative to L32 (primers: TLR5 forward GCA GGA TCA TGG CAT GTC AAC; TLR5 reverse ATC TGG GTG AGG TTA CAG CCT). (B) and (C) Small intestinal TLR5 mRNA levels were quantified by qRT-PCR relative to L32 in female Tlr2-/- (N = 7 mice per group) and Tlr4-/- C57BL/6J mice (N = 7 mice per group). Results are shown as means +− s.e.m. ns = not significant; independent samples Student's t-test.