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. 2015 Apr 29;6(3):202–206. doi: 10.1080/19490976.2015.1034417

Figure 3.

Figure 3.

Transcripts of TLR adaptors are changed by the microbiota and MyD88 interferes with TRIF and TOLLIP. (A) Small intestinal mRNA expression levels of the TLR adaptor TOLLIP in 10–14 week old male GF and CONV-R C57BL/6 mice (N = 4–6 mice per group) was quantified by qRT-PCR relative to L32 (primers: Tollip forward CCC AGG ACT TCC TCC GTA TAA; Tollip reverse AGT CT GCC ATA ATT CTT TGC CA). (B) and (C) Small intestinal TOLLIP mRNA levels were quantified by qRT-PCR relative to L32 in female Myd88-/- (N = 7 per group) and Trif-/- C57BL/6J mice (N = 6–7 per group). (D) TRIF mRNA levels quantified by qRT-PCR relative to L32 in female Myd88-/- C57BL/6 mice (N = 7 per group). (E) MyD88 mRNA levels quantified by qRT-PCR relative to L32 in female Trif-/- C57BL/6 mice (N = 7 per group). mRNA levels of the cell cycle marker Cyclin D1 in (F) female Myd88-/- C57BL/6 mice (N = 6–7 per group) and (G) female Trif-/- C57BL/6 mice (N = 7 per group). Results are shown as means +− s.e.m. Two asterisks, P<0.01; 4 asterisks, P<0.0001; independent samples Student's t-test.