Figure 3.

Estrogen-receptor expression is reduced in female SERT⁺ mouse pulmonary artery. ERα and ERβ protein expression in pulmonary artery from female SERT⁺ mice relative to controls (A and B). ESR1 mRNA transcript in whole lung (C and D). Representative blots are shown for ERα (n = 12 pulmonary arteries from 12 animals) and ERβ (n = 9 pulmonary arteries from nine animals) (A and B). All westerns repeated in triplicate; qRT–PCR, n = 9–12 lungs/group. Quantitative data are shown as ±SEM and analysed using an unpaired t-test. *P < 0.05, *P < 0.01 vs. wild-type (WT).