Figure 2.

The processing modes of the microRNA precursors could be partially inferred from degradome sequencing (degradome-seq) data. (A) Schematic diagram of DCL1-mediated “loop-to-base” [first cleave on the “loop” side (green arrows), and then on the “base” side (gray arrows)] processing of a pri-miRNA (primary microRNAs). The poly(A)-tailed cleavage remnants generated from the first and the second steps of DCL1-mediated cropping could be detected by degradome-seq. (B) Schematic diagram of DCL1-mediated “base-to-loop” [first cleave on the “base” side (green arrows), and then on the “loop” side (gray arrows)] processing of a pri-miRNA. The poly(A)-tailed processing intermediates generated from the first cleavages on the “base” side could be detected by degradome-seq.