Figure 3.

Oligomerization of HuR RRM3. (A) Sedimentation equilibrium measurements of RRM3. The apparent molecular weight (MW) is determined as 19033 ± 1425 Da, larger than the expected for a monomer (14342.2 Da). (B) Sedimentation velocity measurements on RRM3 (S is the sedimentation coefficient). (C) Sedimentation velocity measurements on RRM3 W261E done as in (B). (D) Overlay of the¹H-¹⁵N-HSQC spectra of RRM3 WT (gray) and RRM3 W261E (black). The resonances of the W261-T271 segment are labeled. A proline residue is at position 266. (E) Comparison of the CS23D structural models of RRM3 WT (light gray) and RRM3 W261E (dark gray). The RMSD for backbone atoms between both models is 1.5 Å, being the main differences at the β₂-β₃ and α₂-β₄ flexible loops. The structure views are rotated 180° around the vertical axis.