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. 2015 Oct 23;7:18. doi: 10.3389/fnsyn.2015.00018

Figure 2.

Figure 2

Selective loss of CCK-IR in the mossy fiber (MF) projection pathway of Bassoon mutants. Co-labeling with antibodies against BDNF (red) and CCK (green) revealed a selective loss of CCK-IR in the MF projection pathway of Bsn-mutants. (A) BDNF and (B) CCK-IR in the dentate gyrus (DG) (upper images) and CA3b (lower images) regions of an adult WT mouse. (C) Merged image of BDNF/CCK-IR reveals segregation of the two neuropeptide signals throughout the DG and CA3. Whereas the intensity of (D) BDNF-IR is elevated in the Bsn-mutant hilus and SL, there is a dramatic loss of (E) CCK-IR in granule cell axon terminals (i.e., MFBs) in the hilus and SL. (F) Merged image of BDNF/CCK-IR emphasizes upregulation of BDNF-IR, and corresponding down-regulation of CCK-IR in the hilus and SL of Bsn-mutants. Nuclei are labeled with DAPI (blue). Scale bar: 100 μm. Abbreviations: IML, inner molecular layer; GCL, granule cell layer; H, hilus; DG, dentate gyrus; SL, Stratum Lucidum; SP, Stratum Pyramidale.