Skip to main content
. 2015 Oct 23;7:18. doi: 10.3389/fnsyn.2015.00018

Figure 3.

Figure 3

Loss of Calbindin immunoreactivity in Bsn-mutant dentate granule cells. (A) Normal distribution of CB-IR in the WT DG. Note uniform distribution of immunoreactivity throughout the granule cell bodies and their associated dendrites and MFs in the ML and hilus, respectively. (B) Loss of CB-IR in granule cells, dendrites and MFs in the Bsn-mutant DG. Note that the outer GCL is particularly affected by this loss. (C,D) Higher magnification view of the GCL and ML in WT vs. Bsn-mutants. (E) Strong, uniform CB-IR in MFs contained within SL of hippocampal area CA3. Comparison with CB-IR in the (F) Bsn-mutant reveals a downregulation in staining intensity (SI), indicating reduced CB protein content in MFs. Scale bars: A,B,E,F = 100 μm; C,D = 20 μm. Abbreviations: ML, molecular layer; GCL, granule cell layer; h, hilus; SL, stratum lucidum.