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. 2015 Oct 23;7:18. doi: 10.3389/fnsyn.2015.00018

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Copyright © 2015 Dieni, Nestel, Sibbe, Frotscher and Hellwig.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BDNF-positive DCVs accumulate at the synaptic membrane in MFBs from Bsn-mutant mice. Immunogold-labeled MFB showing the relative position of BDNF+ DCVs (white asterisks) and the MFB terminal membrane (arrows) in (A) WT mice and (B) Bsn-mutants. (C) Quantification of vesicle distance from the membrane revealed that BDNF+ DCVs accumulate closer to the synaptic membrane in Bsn-mutants compared to WT mice. Data are shown as mean ± SEM, circles represent single data points. *p < 0.05. Scale bar = 200 nm. Abbreviations: MFB, mossy fiber bouton.