Figure 4.

Knockdown activity and processing of AgoshPol47 variants. (A) The knockdown activity of the guide strand on Luc-sense (upper panel) and passenger strand on Luc-antisense (lower panel) of the AgoshPol47 was determined by co-transfection of a luciferase reporter encoding the sense and antisense target sequence, respectively, in HEK293T cells. We performed 3 independent transfections, each in duplicate, and standard deviations were calculated. (B) Total RNA was analyzed by northern blot for processing products derived from the 3′ strand (upper panel) and 5′ strand (lower panel). Size markers are indicated on the right. The regular shRNA ∼21 nt products are marked and * indicates the AgoshRNA ∼30 nt products. See Figure 3 for more details.