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Immunohistochemical staining. Representative images of the immunohistochemical staining of BDNF, p-Akt, Bcl-2, and Bax in area CA1 of the hippocampus. Cells with a brown-stained cytoplasm are considered positive. Sham, sham-operated group; VaD, VaD model group; Cb H, C. butyricum- (1 × 108 CFU-) treated group; Cb M, C. butyricum- (1 × 107 CFU-) treated group; and Cb L, C. butyricum- (1 × 106 CFU-) treated group. Magnification: 400x. Scale bar = 20 μm.
