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Purification of PulN by Ni-NTA affinity chromatography
| Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification fold |
|---|---|---|---|---|---|
| Crude extract | 292.05 | 77.26 | 3.78 | 100.00 | 1.00 |
| Ni-NTA | 171.41 | 10.60 | 16.17 | 58.7 | 4.28 |
The crude enzyme was obtained from 45 ml culture and 10 ml purified enzyme was obtained.
