Fig. 1.

Inhibition of mitochondrial complex I with BAY 87-2243 in melanoma cells induces cell death in vitro and reduces tumor growth in vivo. a BRAF wild type and mutant melanoma cells were treated with various concentrations of BAY 87-2243. Cell viability was measured after 72 h. IC50 values were calculated using GraphPad prism (n = 4). b Melanoma cells were treated with BAY 87-2243 (10 nM). Cell death was measured after 72 h using propidium iodine (n = 4). c Melanoma cells were treated with BAY 87-2243 (10 nM). Cell viability was measured after 24, 48, and 72 h (n = 3). d Nude mice were xenotransplanted with either 3 × 10⁶ SK-MEL-28 (50 % matrigel) or 5 × 10⁶ G-361 (100 % matrigel) human melanoma cells (n = 10 per group). When tumor area reached around 50 mm², mice were treated once a day with vehicle or 9 mg/kg BAY 87-2243 per oral gavage (p.o.). e Nude mice bearing patient-derived (MEXF 276, MEXF 1732, n = 8 per group) melanoma xenograft tumors were treated orally (p.o.), once a day with vehicle or 9 mg/kg BAY 87-2243. Data are represented as the mean ± SD. *p < 0.05