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. 2015 Oct 20;12:171. doi: 10.1186/s12985-015-0376-3

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© Xiao et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — RT-PCR detection of GRSPaV from RNAs isolated with Spectrum™ Plant Total RNA kit (Sigma). a Agarose gel analysis of RT-PCR products with primers RSP21 and RSP22 targeting the capsid protein gene (Additional file 1: Table S1) on total RNA extracts from V. vinifera var. Chardonnay (lane 1), V. riparia from Quebec (lane 2), V. riparia from Manitoba (lane 3), V. vinifera var. Riesling (lane 4) and V. vinifera var. Thompson seedless (lane 5). Lane M: molecular size marker (bp); lane 6: water control. b Agarose gel analysis of RT-PCR products using total RNA extracts from V. vinifera var. Syrah. Lanes 1–6: leaf samples from six individual vines. Lane 7: water. Lane M: molecular size marker (bp)