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. 2015 Mar 25;12(4):389–397. doi: 10.1080/15476286.2015.1026033

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

© Francesco Severi and Silvestro G Conticello

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — The amount of RNA editing depends on the exposure of the chimeric transcript to APOBEC1. HuH-7 cells were transiently transfected with the double-fluorescent chimera and the indicated plasmids. (A) RNA editing (percentage of gated cells) at different time points (hours) after transfection. (B) Titration of RNA editing in cells transiently transfected with different amounts of APOBEC1 expressing plasmid or with control plasmids. (C) The bar diagram shows the RNA editing in HuH-7 transiently cotransfected with plasmids encoding for either rat, human, lizard APOBEC1 or a chimera of lizard APOBEC1 fused with the C-terminal portion of the rat APOBEC1. The error bars represent the SEM from at least 3 experiments.