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. 2015 Jul 28;6(4):289–300. doi: 10.1080/19491034.2015.1074359

Figure 1.

Figure 1.

Proteins encoded by the BPV1 E2 ORF have different subnuclear localisations. (A) A schematic representation of the E2 proteins found in BPV1-transformed cells. The location of the E8 ORF with respect to the E2 ORF is not drawn to scale. The ATG of the E8 ORF (at nt 1204) is located 404 bp upstream from the E2 ATG (at nt 2608). (B) The biochemical fractionation protocol. (C) All 3 E2 proteins localized to the cell nucleus in transiently transfected CHO cells in an IF analysis. (D) Western blot of the biochemical fractionation profiles of different E2 proteins in transiently transfected CHO cells.