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The E8 part of the E8/E2 protein is required for NM targeting. Western blot of biochemical fractions from CHO and Cos7 cells transfected with respective plasmids. The differences between the expression constructs are depicted on the right. The arrowheads mark the transcription start site. The asterisk indicates the point mutation K2A. Sol - CSK soluble fraction, 0.4 - the soluble fraction in CSK + 0.4 M NaCl, P - the insoluble fraction in CSK + 0.4 M NaCl.
