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. 2015 Oct 3;5:824–831. doi: 10.1016/j.fob.2015.09.009

Table 1.

Steady-state kinetic parameters and specificity constants.

Enzyme kcat (s−1) KmGly (mM) KmSCoA (μM) kcat/KmGly (s−1 M−1) kcat/KmSCoA (s−1 M−1)
wt mALAS2 0.25 ± 0.004 8 ± 0.7 1.0 ± 0.1 31.2 ± 3.0 2.5 × 105 ± 0.3 × 105
K221V 0.17 ± 0.002 50 ± 13 23 ± 2 3.4 ± 0.7 0.07 × 105 ± 0.007 × 105