Fig. 4.

SUSD4a expressing BT20 cells form smaller colonies. The clonogenic potential of BT20 cells expressing SUSD4a was tested in co-culture with fibroblasts. SUSD4a-transfected BT20 cells formed significantly smaller colonies in co-culture with CAFs (a) but not with control fibroblasts (b). The ability of the SUSD4a-transfected cells to adhere to CAFs was also tested. No significant differences were observed for either MDA-MB-231 (c) or BT20 (d). The graphs represent data collected from at least three independent experiments ± SD; ns and ** stand for not significant and p < 0.01, respectively