Figure 6. Multi-omics comparison of the impact of puf3∆ on gene regulation.

Hierarchical clustering analyses of log₂ fold changes from each of our ‘omics datasets analysed in Figures 3, 4, 5. 1798 genes are represented across the 6 data sets. Genes are depicted as coloured vertical lines representing membership of the four Puf3p interacting groups (Core, RSU, PCU and non-target mRNAs). Purple boxes delineate 6 gene clusters (I-VI) with similar profiles. Pie charts show proportional group membership in each cluster. Groups statistically over-represented (P < 0.001, Chi squared test) in each cluster are named beneath each pie. Lower panel, GO categories showing a group association (χ² test for independence; Bonferroni corrected p-values < .01). Colours show if the GO terms are significantly enriched (red) or depleted (blue) within the groups. White colour indicates non-significant enrichments/depletions. See Supplementary Dataset S1 for a list of genes in each cluster.