Figure 2. Motion scores of chick embryo movement as a function of time for three anesthesia regimen, medetomidine at 0.3 mg/kg, ketamine/midazolam at 50 mg/kg and 1 mg/kg, and thiopental at 100 mg/kg, respectively (n = 7).

Scores were assigned during each 4 min of videotaping, immediately prior anesthesia (0 min), and every 10 min during anesthesia for one hour and immediately after antagonization where applicable (70 min). Medetomidine is considered the most suitable of the different anesthesia regimen studied for the purpose to assess perfusion capacity of biomaterials grown on the CAM non-destructively in ovo using contrast-enhanced MRI and during the required time frame of 30 minutes (dashed blue lines). Error bars denote standard errors.