Figure 2. Agarose gel electrophoresis for all four types of DNA origami structures.

Fold = DNA structures after folding in the folding buffer (FOB) (for ST and DT FOB is 1× TAE + 12.5 mM Mg²⁺ and for HT and HB 1× TAE + 20 mM Mg²⁺). Filt = DNA structures filtered with pure water. Filt+FOB = Filtered DNA structures stored in water for 1 day, after which the buffer conditions have been adjusted to the same as for folding. S is a scaffold strand (M13mp18), which is used as a reference sample.