Figure 4. Patterning substrates with fluorescently labelled DNA origamis.

(a) A photograph of a mechanical polydimethylsiloxane (PDMS) mask (with round 1.8 mm diameter openings) on top of the glass substrate. (b) A schematic view of the patterning setup. Spray-coating of labelled DNA origamis is carried out through the PDMS mask. (c) Fluorescence micrographs of patterns created using sequential spray-coating of Cy5-labelled and purified 60-helix bundles (HB) onto a glass substrate. HBs were deposited first through a mask with large holes (1.8 mm in diameter, blue dashed circle) and after that through a mask having slightly smaller holes (1 mm in diameter, yellow dashed circle). The scale bars are 1 mm. (d) Fluorescence micrographs of the glass substrates after coating with Cy5-labelled and purified HBs (left) and hexagonal tubes (right). Micrographs in (d) were taken after the mask removal at the edges of the formed round patterns (diameter 1.8 mm) as indicated by the black square and the dashed lines. The scale bars are 50 μm.