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. 2015 Oct 20;6:8608. doi: 10.1038/ncomms9608

Figure 8. TFR impair TFH cytokine production.

Figure 8

(a) Tonsil TFH were analysed by intracellular cytokine staining for IL-4 and IL-21 production following mock-, X4- or R5-spinoculation. (b) CD25+ regulatory cells were depleted before spinoculation and IL-4 (n=3) and IL-21 (n=7) production by TFH cells were analysed after 2 days. (c) IL-4 and IL-21 production by TFH were analysed in disaggregated lymph node cells from rhesus macaques with and without CD25-depletion (n=3). (d) Sorted populations of tonsil TFH and TFR were spinoculated, TFR were added back to TFH at an increasing ratio, and TFH production of IL-4 and IL-21 were measured by intracellular cytokine assays at day 2 (n=4). (e) Sorted tonsil TFH and TFR were cultured for 2 days in the presence of IL-10- and TGF-β-neutralizing antibodies and TFH production of IL-21 was analysed (n=2). Statistical analyses were performed by Friedman nonparametric tests (a,b) and significance is denoted by asterisks where *P<0.05 and ***P<0.001.