Figure 3.

Neutrophils use VLA-4 integrin when recruited by VEGF-A. The role of the 3 major leukocyte integrins (LFA-1, Mac-1, and VLA-4) in neutrophil recruitment by VEGF-A was accessed by intravital fluorescence video microscopy of the cremaster muscle of mice pretreated with neutralizing mAbs directed to CD11a, CD11b, or CD49d. Neutrophils were immunostained in vivo with Ly6G (clone 1A8) mAb conjugated to Alexa Fluor Dye 555 prior to the experiment. The number of (A) adherent and (B) emigrated neutrophils, as well as the fraction of intravascular (C) crawling and (D) detaching neutrophils upon 90 min of stimulation with VEGF-A (n = 4-5 mice per treatment). *P < .05 compared with other groups, as indicated.