Gene copies of antibiotic resistance genes (ARGs) normalized to 16S rRNA. The error bars are the standard variations for triplicate samples. The tetracycline-related ARGs include tet(M), tet(O), tet(Q), and tet(W), the sulfonamide-related resistance genes include sul1 and sul2, and the quinolone-related ARGs include qnrA, qnrB, and qnrS. Refer to the legend to Fig. 1 for sample designations.