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Analysis of bioconverted product by HPLC. The supernatant fluid from the indicated mixtures incubated at 28°C for 4 h was analyzed by HPLC using a Senshu Pak SCX-1251-N column. (a) Authentic d-CS. (b) Cells of E. coli BL21(DE3) harboring pET-21a(+) with l-serine and HU (each at 2.5 mM). (c) Cells of E. coli BL21(DE3) harboring pET-21a(+)/dcsEDCG with l-serine (2.5 mM). (d) Cells of E. coli BL21(DE3) harboring pET-21a(+)/dcsEDCG with l-serine and HU (each at 2.5 mM).
