. 2015 Oct 21;81(22):7881–7887. doi: 10.1128/AEM.02187-15

TABLE 1.

Primers used in this study

Name	Sequence (5′→3′)
dcsE_F	CATATGAGGGAATTCATACCCCCGGCCTCC
dcsE_R	AAGCTTGGACTAGTTCAGGCGACGATCGCCAGGAACTTCG
dcsD_F	CATATGCCTCTGTTTAACAGCATCCTCGACACCATC
dcsD_R	AAGCTTGGACTAGTCTAGAGGCCGGAGCCGGTGTC
dcsC_F	CATATGATACGCATGAGAACGCCGAGCACG
dcsC_R	AAGCTTGGACTAGTTTAAACAGAGGCATGGAGGAAGGTTCCTTCG
dcsG_F	CATATGGGCATCCTCGCCTTGGTCACC
dcsG_R	AAGCTTGGACTAGTCTAGGGCTTGAGCCGTTCGGC
dcsJ_F	CATATGGATGCACGACACAGCACGATCACGTTGA
dcsJ_R	AAGCTTGGACTAGTCTACGCTCTCGCCCGCTGCGT
cysJ_U_F	GAATTCTTTACCCACCAGGCCCACTTCCGCCA
cysJ_U_R	TCTAGAGAGCGCCGTTATCAGCGAGATGTCTAC
cysJ_D_F	CTGCAGCGGAAGGTGGGACCTGTGTCGTCAT
cysJ_D_R	GCATGCCACCAGATGCGCACCATACTCCAGTGATTC
Tet^r_F	TCTAGAAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCT
Tet^r_R	CTGCAGGTCGCAGACGTTTTGCAGCAGCAGTC
cysJ_dis_F	ATGATCAGATTCTGGTTCGCCGTAATGCGGA
cysJ_dis_R	CGGAGAATATCGTCACCACAAATAACGCCACA
cysJ_check_F	CATCTCCTTGCATGCACCATTCCTTGC
cysJ_check_R	GCCGTGACGACGTAAATGATGCCAGAAT
cysK_U_F	GAATTCCGAACATATCCTAACTGTCCATTGCGCAATTACCC
cysK_U_R	AAGCTTCTCCGGACCGGTGGTTTTTTCGTGAATTTCAG
cysK_D_F	GCATGCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGC
cysK_D_R	GTCGACCTCTGCACCTTCAACGTCACGAACCGTA
Km^r_F	AAGCTTTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
Km^r_R	GCATGCTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATA
cysK_dis_F	CTAACTGTCCATTGCGCAATTACCCGGT
cysK_dis_R	CGTACCAATGTTAGCGCATACTTCTACCTG
cysK_check_F	CCGATGCGCCAGAGTTGTTTCTGAAACA
cysK_check_R	CGTCGCGCAGTTCCTGTTTGTAGATTTC