TABLE 2.
Plasmids used in this study
| Plasmid name | Purpose | Source or reference |
|---|---|---|
| pKFT | Genetic manipulation | 17 |
| pKFT (sarA FR) | Genetic manipulation | This study |
| pFK25 (pKFT (sarS FR)) | Genetic manipulation | 17 |
| pKFT (sarT FR) | Genetic manipulation | Sugai laboratory |
| pKFT (sarU FR) | Genetic manipulation | Sugai laboratory |
| pKFT (rot FR) | Genetic manipulation | In this study |
| pKFT (saeR FR) | Genetic manipulation | Sugai laboratory |
| pKAT | Genetic manipulation | Sugai laboratory |
| pKAT::sarA | Genetic manipulation | Sugai laboratory |
| pKAT::sarT | Genetic manipulation | Sugai laboratory |
| pKAT::sarU | Genetic manipulation | Sugai laboratory |
| pKAT::rot | Genetic manipulation | This study |
| pWH1520 | Genetic manipulation | MoBiTec |
| pWH1520::sarS | Genetic manipulation | 18 |
| pWH1520::saeR | Genetic manipulation | Sugai laboratory |
| pGEM-T Easy | EMSA | Promega |
| pGEM-T Easy/SEH promoter | EMSA | This study |
| pET28a | EMSA | Novagen |
| pGrota | EMSA | 20 |
a
Reconstructed in this study.