TABLE 3.
Primers used in this study
| Primer name | Sequence (5′-3′) | Purpose |
|---|---|---|
| gyrB forward | AGGTCTTGGAGAAATGAATG | qPCR |
| gyrB reverse | CAAATGTTTGGTCCGCTT | qPCR |
| seh forward | TCAAGGTGATAGTGGCAAT | qPCR |
| seh reverse | CCAATCACCCTTTCCTGT | qPCR |
| rot forward | TGCAGTATTTCAACCACACAC | qPCR |
| rot reverse | GTATCGTTAATGCGCCAGT | qPCR |
| M13 forward | GTAAAACGACGGCCAGT | Genetic manipulation |
| M13 reverse | CAGGAAACAGCTATGAC | Genetic manipulation |
| SarA1 | AGGATCCAAAAATTCTAATGGA | Genetic manipulation |
| SarA2 | GGATCCTCAACTCATTCTTAAG | Genetic manipulation |
| SarA3 | GGTTTTGCACTAATGGCACT | Genetic manipulation |
| SarA4 | TTGCTCGATACATTTGCCCGA | Genetic manipulation |
| sarS1 | GCCAAAGCTTATACATGGCTAGTCGG | Genetic manipulation |
| sarS4 | TCAAGGATCCATAGAAGGCGCTTTG | Genetic manipulation |
| sarT F-F | GGCTGCAGGATAGTGACTTAATGTTCTT | Genetic manipulation |
| sarT F-R | CCGGATCCGTCTTCATCCCTTACTTTTAA | Genetic manipulation |
| sarT R-F | GGGGATCCAGTATGTTTCGAGATTTTAAT | Genetic manipulation |
| sarT R-R | CCGAGCTCTACACCCTGTGGTGCAGTGTC | Genetic manipulation |
| sarT F | CGGGATCCCGTTATGTTTCATTAATATTTATTTC | Genetic manipulation |
| sarT R | CCCAAGCTTGGGCCTTACATTCTCCTACTA | Genetic manipulation |
| sarU F-F | CGGGATCCCGTTATGTTTCATTAATATTTATTTC | Genetic manipulation |
| sarU F-R | GGCTGCAGCTTACATTCTCCTACTACTATTTTC | Genetic manipulation |
| sarU R-F | GGCTGCAGATTTAACAGATTTACCTCTTG | Genetic manipulation |
| sarU R-R | CCCAAGCTTGTGACGATATTGTTGAATCTG | Genetic manipulation |
| sarU F | GGGTTCGAAGTCTTCATCCCTTACTTTTAA | Genetic manipulation |
| sarU R | CCCAAGCTTTTAAAAGAAAAATTTTCTTGG | Genetic manipulation |
| rot1 | AATGGATCCACAAGGTATTA | Genetic manipulation |
| rot2 | GGGGATCCTGTTAATTTCTCCT | Genetic manipulation |
| rot3 | TCAAATGTTGACTACTCAAT | Genetic manipulation |
| rot4 | ATAAACTTGCTTTCTATTCA | Genetic manipulation |
| rot comp S | GCCGAAGCTTTAAGGTTGAGAATGTATATC | Genetic manipulation |
| rot comp AS | GCCGAAGCTTTTACACAGCAATAATTGCGT | Genetic manipulation |
| SaeF | TGGAAAGCTTATGATTTCACAGCACCC | Genetic manipulation |
| SaeSR | TTGCAAGCTTGATACAAGTAATTGGTC | Genetic manipulation |
| T7 promoter-1 | TAATACGACTCACTATAGGG | EMSA |
| T7 promoter-1 cy3a | TAATACGACTCACTATAGGG | EMSA |
| SP6 promoter-1 | CAAGCTATTTAGGTGACACTATAG | EMSA |
| SEH upstream S | TCTAACTACTATAGCAACTG | EMSA |
| SEH upstream AS | TTTAAAACTCCTCAATATAT | EMSA |
| g-rot-f-NcoI | GCGCCATGGTGAAAAAAGTAAATAACGACACT | EMSA |
| g-rot-r-XhoI | GCGCTCGAGCACAGCAATAATTGCGTTTA | EMSA |
a
cy3 labeled at the 5′ end.