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Effect of pH, ionic strength, and temperature on the stability of virus-like particle tertiary structure, as monitored by UV fluorescence emission
| Condition | Tryptophan emission peak (nm) |
||
|---|---|---|---|
| GI.1 | GII.4 | FCV | |
| pH | |||
| 3 | 331.4 | 329.7 | 329.0 |
| 7 | 332.4 | 328.7 | 329.7 |
| 8 | 333.4 | 329.4 | 330.7 |
| Ionic strength (M) | |||
| 0 | 332.05 | 331.0 | 330.0 |
| 0.1 | 333.0 | 330.4 | 330.0 |
| 0.25 | 332.4 | 329.0 | 331.0 |
| Temp (°C) | |||
| 4 | 334.7 | 328.7 | 330.3 |
| 22 | 332.4 | 328.7 | 329.7 |
| 65 | 335.0 | 330.0 | 333.0 |
| 75 | 338.7 | 335.0 | 335.0 |
