Coexpression of subtilin variants. Shown are comparative RP-HPLC chromatograms of subtilin variant producers and wild-type entianin producer B15029. The y axes of all chromatograms display the same intercepts. Greek letters indicate the corresponding lantibiotic, verified by mass spectrometry analysis. S-subtilin, succinylated lantibiotic molecules. (A) RP-HPLC chromatograms of B.15029.TSp66 ΔetnS spaS (W1A), B15029 entS+, and B15029.TSp91 etnS+
spaS (W1A). (B) RP-HPLC chromatograms of B15029.TSp57 ΔetnS spaS (W1E), B15029 etnS+, and B15029.TSp89 etnS+
spaS (W1E). (C) RP-HPLC chromatograms of B15029.TSp71 ΔetnS spaS (W1K), B15029 etnS+, and B15029.TSp98 etnS+
spaS (W1K). mAU, milli-absorbance units.