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. 2015 Oct 21;5:238. doi: 10.3389/fonc.2015.00238

Figure 2.

Figure 2

Effect of soy and radiation on connective tissue layers of esophagus. Esophageal sections obtained from the experiments described in Figure 1 were stained with Masson’s Trichrome for detection of collagen fibers within the LP and SM connective tissues. Insets 1 and 2 show normal esophagi with collagen staining in LP and SM below the negative staining of MM. (A) Sections from control mice showed similar collagen fibers distribution limited to LP and SM as labeled in A1. (B) Sections from mice treated with soy showed staining patterns comparable to controls. (C) Sections from mice treated with radiation showed disruptions in the LP (labeled in C1) and infiltration of collagen fibers focally into the MM with entrapment of hypertrophic smooth muscle cells (arrows in C2). (D) Sections from mice treated with soy + radiation showed reduced collagen infiltration into MM smooth muscle compared to radiation and a higher density of fibers (labeled in D1) that is comparable to LP of control and soy-treated esophagus. All magnifications ×100.