Figure 2.

Live/dead staining showed a high cell viability of fetal hMSCs after 9 days of static culture in a well plate (A,B) and after 3 days of culture in static condition in a plate followed by 6 days of culture in a biaxial bioreactor (C,D). Higher numbers of cells were found in the scaffolds after culture in the biaxial bioreactor compared to the scaffolds from static culture. This was confirmed by DNA quantification (E) where a significant difference in cell number was found between static culture in a plate and culture in the biaxial bioreactor. DNA quantification also shows a significance increase in cell number in time (90 min, 3, 9 days) when the cells are cultured statically in a well plate. (F) Collagen production per cell did not increase in time and significantly decreased upon culture in the biaxial bioreactor compared to 1 day of static culture subsequent to 90 min of seeding in a concentrated suspension and to 3 days of static culture [90 min and 6 h (n = 5), day 3 (n = 10), day 9 static (n = 12), day 9 biaxial bioreactor (n = 14), *p < 0.05, **p < 0.01, ***p < 0.001].